Expression of SANT/HTH Myb mRNA, a plant morphogenesis-regulating transcription factor, changes due to viroid infection.

Autor: Matoušek J; BC ASCR v. v. i., Institute of Plant Molecular Biology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: jmat@umbr.cas.cz., Piernikarczyk RJ; Institute of Physical Biology, Heinrich-Heine-Universität Düsseldorf, D-40204 Düsseldorf, Germany. Electronic address: Rajen.Piernikarczyk@uni-duesseldorf.de., Týcová A; BC ASCR v. v. i., Institute of Plant Molecular Biology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: tycova@umbr.cas.cz., Duraisamy GS; BC ASCR v. v. i., Institute of Plant Molecular Biology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: gansels@umbr.cas.cz., Kocábek T; BC ASCR v. v. i., Institute of Plant Molecular Biology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: kocabek@umbr.cas.cz., Steger G; Institute of Physical Biology, Heinrich-Heine-Universität Düsseldorf, D-40204 Düsseldorf, Germany. Electronic address: steger@biophys.uni-duesseldorf.de.
Jazyk: angličtina
Zdroj: Journal of plant physiology [J Plant Physiol] 2015 Jul 01; Vol. 183, pp. 85-94. Date of Electronic Publication: 2015 Jun 09.
DOI: 10.1016/j.jplph.2015.06.001
Abstrakt: Potato spindle tuber viroid (PSTVd) belongs to plant-pathogenic, circular, non-coding RNAs. Its propagation is accompanied by (mis)regulation of host genes and induction of pathogenesis symptoms including changes of leaf morphogenesis depending on the strength of viroid variant. We found strong genotype-dependent suppression of tomato morphogenesis-regulating transcription factor SANT/HTH-Myb (SlMyb) due to viroid pathogenesis. Its relative mRNA level was found to be significantly decreased in PSTVd-sensitive tomato (cvs Rutgers and Heinz 1706) due to degradation processes, but increased in PSTVd-tolerant (cv. Harzfeuer). In heterologous system of Nicotiana benthamiana, we observed a SlMyb-associated necrotic effect in agroinfiltrated leaf sectors during ectopic overexpression. Leaf sector necroses were accompanied by activation of nucleolytic enzymes but were suppressed by a strongly pathogenic PSTVd variant. Contrary to that, PSTVd's effect was inhibited by the silencing suppressor p19. It was found that in both, Solanum lycopersicum leaves and N. benthamiana leaf sectors, SlMyb mRNA degradation was significantly stronger in viroid-infected tissues. Necroses induction as well as gene silencing experiments using the SANT/HTH-Myb homologues revealed involvement of this Myb in physiological changes like distortions in flower morphogenesis and growth suppression.
(Copyright © 2015 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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