Effect of Mucosal Cytokine Administration on Selective Expansion of Vaginal Dendritic Cells to Support Nanoparticle Transport.

Autor: Ramanathan R; Department of Bioengineering, University of Washington, Seattle, WA, USA., Park J; Department of Bioengineering, University of Washington, Seattle, WA, USA., Hughes SM; Department of Obstetrics and Gynecology, University of Washington, Seattle, WA, USA., Lykins WR; Department of Bioengineering, University of Washington, Seattle, WA, USA., Bennett HR; Department of Bioengineering, University of Washington, Seattle, WA, USA., Hladik F; Department of Obstetrics and Gynecology, University of Washington, Seattle, WA, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.; Department of Medicine, University of Washington, Seattle, WA, USA., Woodrow KA; Department of Bioengineering, University of Washington, Seattle, WA, USA.
Jazyk: angličtina
Zdroj: American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2015 Oct; Vol. 74 (4), pp. 333-44. Date of Electronic Publication: 2015 Jun 27.
DOI: 10.1111/aji.12409
Abstrakt: Problem: The capacity of antigen-carrying vaccine nanoparticles (NPs) administered vaginally to stimulate local immune responses may be limited by the relatively low numbers of antigen-presenting cells (APCs) in the genital mucosa. Because inflammation is associated with increased susceptibility to sexually transmitted infections, we sought to increase APC numbers without causing inflammation.
Method of Study: In this study, we evaluated intravaginal delivery of chemokines, growth factors, or synthetic adjuvants to expand APCs in reproductive tissues.
Results: We found that granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulated expansion of CD11b+ dendritic cells (DCs) within 24 hr of intravaginal administration, with no effect on Langerhans cells or macrophages. Expansion of the CD11b+ DC population was not associated with increased inflammatory cytokine production, and these cells retained phagocytic function.
Conclusion: Our data suggest that non-inflammatory expansion of mucosal APCs by intravaginal GM-CSF could be used as an adjuvanting strategy to potentiate the genital immune response to nanoparticulate mucosal vaccines.
(© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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