Host Defense Proteins in Breast Milk and Neonatal Yeast Colonization.

Autor:	Chow BD; Marshfield Clinic Research Foundation, Marshfield, WI, USA., Reardon JL; Brown University, Providence, RI, USA., Perry EO; Bristol County Agricultural High School, Dighton, MA, USA., Laforce-Nesbitt SS; Brown University, Providence, RI, USA Women & Infants Hospital of Rhode Island, Providence, RI, USA., Tucker R; Women & Infants Hospital of Rhode Island, Providence, RI, USA., Bliss JM; Brown University, Providence, RI, USA Women & Infants Hospital of Rhode Island, Providence, RI, USA jbliss@wihri.org.
Jazyk:	angličtina
Zdroj:	Journal of human lactation : official journal of International Lactation Consultant Association [J Hum Lact] 2016 Feb; Vol. 32 (1), pp. 168-73. Date of Electronic Publication: 2015 Jun 26.
DOI:	10.1177/0890334415592402
Abstrakt:	Background: Colonization increases risk for invasive candidiasis in neonates. Breast milk host defense proteins may affect yeast colonization of infants. Objective: This study aimed to evaluate breast milk host defense proteins relative to yeast colonization in infants. Methods: Infants admitted for longer than 72 hours to the neonatal intensive care unit at Women & Infants Hospital in Providence, Rhode Island, were eligible. After consent, expressed breast milk and swabs from oral, rectal, and inguinal sites from infants were cultured weekly for 12 weeks, or until discharge, transfer, or death. Breast milk was tested for levels of human lactoferrin, lysozyme, apolipoprotein J, mucin-1, dermcidin, and soluble CD14 using commercial ELISA. Concentrations of these components were compared in breast milk received by infants who were colonized or not colonized with yeast. Results: From an original cohort of 130, 61 infants had samples available for this subanalysis. A convenience sample of stored breast milk was analyzed. Median lactoferrin, apolipoprotein J, and mucin-1 did not differ between colonized and uncolonized groups. Soluble CD14 was higher in the surface-colonized group (1.8 μg/mL, n = 12) compared with the surface-uncolonized group (1.6 μg/mL, n = 12, P = .02). Median lysozyme levels were higher in the surface-uncolonized group (483.0 ng/mL, n = 12) versus the surface-colonized group (298.3 ng/mL, n = 12, P = .04). Median dermcidin levels were higher in the surface-uncolonized group (19.4 ng/mL, n = 12) versus the surface-colonized group (8.7 ng/mL, n = 12, P = .04). Conclusion: This study shows an association between colonization with Candida in neonates and lower levels of lysozyme and dermcidin in received breast milk. Further study is needed to confirm these findings. (© The Author(s) 2015.)
Databáze:	MEDLINE
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