| Autor: |
Mason TM; Department of Experimental Haematology, Christie Hospital and Holt Radium Institute, Withington, Manchester., Lord BI, Hendry JH |
| Jazyk: |
angličtina |
| Zdroj: |
British journal of haematology [Br J Haematol] 1989 Dec; Vol. 73 (4), pp. 455-61. |
| DOI: |
10.1111/j.1365-2141.1989.tb00280.x |
| Abstrakt: |
The radial distributions of spleen colony forming units (CFU-S) and in-vitro colony forming cells (in-vitro-CFC) were measured in the diaphyseal marrow cavity of femora removed from 3-, 5- and 11-week-old mice. The distributions observed in 11-week-old mice confirm earlier findings that the highest concentrations of CFU-S exist near bone surfaces whereas the concentration of in-vitro-CFC increases to a peak value approximately 300 microns from the femoral axis with a low value at the bone surface. The gradients of the distributions in all three age groups are very similar suggesting that spatial organization in marrow is established by 3 weeks at the latest and, as the marrow cavities grow, so the distributions extend into the new space following their respective gradients. The peak of CFU-S concentration at the bone surfaces in all age groups coincides with increased rates of DNA synthesis and a low self-renewal capacity. Conversely, CFU-S nearer the centre of the cavity maintain a low turnover but have a high self-renewal capacity. Measurements made on 1-week-old mice show that the marrow contains a lower average concentration of CFU-S in the femoral cavity compared to older mice. However, these CFU-S have both a high rate of turnover and a high self-renewal capacity. It appears that these better quality CFU-S remain in a central location while the rest of the population ages and expands in association with growing bone regions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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