Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coli in Retail Meats and Shrimp at a Local Market in Vietnam.

Autor:	Le QP; 1 Nha Trang Pasteur Institute , Nha Trang, Vietnam ., Ueda S; 2 University of Ryukyus , Okinawa, Japan ., Nguyen TN; 1 Nha Trang Pasteur Institute , Nha Trang, Vietnam ., Dao TV; 1 Nha Trang Pasteur Institute , Nha Trang, Vietnam ., Van Hoang TA; 1 Nha Trang Pasteur Institute , Nha Trang, Vietnam ., Tran TT; 1 Nha Trang Pasteur Institute , Nha Trang, Vietnam ., Hirai I; 2 University of Ryukyus , Okinawa, Japan ., Nakayama T; 3 Osaka University , Osaka, Japan ., Kawahara R; 4 Osaka Prefectural Institute of Public Health , Osaka, Japan ., Do TH; 1 Nha Trang Pasteur Institute , Nha Trang, Vietnam ., Vien QM; 1 Nha Trang Pasteur Institute , Nha Trang, Vietnam ., Yamamoto Y; 3 Osaka University , Osaka, Japan .; 4 Osaka Prefectural Institute of Public Health , Osaka, Japan .
Jazyk:	angličtina
Zdroj:	Foodborne pathogens and disease [Foodborne Pathog Dis] 2015 Aug; Vol. 12 (8), pp. 719-25. Date of Electronic Publication: 2015 Jun 25.
DOI:	10.1089/fpd.2015.1954
Abstrakt:	Background: Contamination of food with multiantibiotic-resistant bacteria, particularly extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, is considered a potential source for the wide dissemination of ESBL-producing bacteria in communities. However, little is known about the extent of contamination of food with ESBL-producing bacteria in Vietnam. Objective: This study was conducted to assess the characteristics of ESBL-producing Escherichia coli isolated from retail meats and shrimp in Nha Trang, Vietnam. Materials and Methods: A total of 350 food samples (poultry [n=143], pork [n=147], and shrimp [n=60]) were purchased in July and November 2013 from a local market. ESBL-producing E. coli were isolated, and ESBL genotypes, phylogenetic groups, and antibiotic resistance profiles were determined. Results: The prevalence of ESBL-producing E. coli in retail foods was 40.6%. β-Lactamase-encoding genes of the CTX-M-1 (50.7%), CTX-M-9 (41.5%), TEM (59.9%), and SHV (2.8%) groups were detected singly or in combination. The percentages of single ESBL isolates harboring CTX-M-1 or -9 plus TEM groups were 35.2% and 16.2%, respectively. B1 was the most prevalent phylogroup in ESBL isolates from pork (44.7%), poultry (55.9%), and shrimp (72.7%). B2 was the least prevalent (4.2% and 4.8% for pork and poultry isolates, respectively). The prevalence of multidrug resistance (MDR; resistance to ≥ 3 antimicrobial groups) in ESBL-producing E. coli isolated from food was 85.9%. Discussion and Conclusions: This is the first report of the characteristics of ESBL-producing E. coli in retail foods in a local city in Vietnam. Our findings indicate that retail foods are contaminated with ESBL-producing E. coli, of which many were MDR. Further monitoring and public health efforts targeting food administration are needed to control the spread of ESBL-producing bacteria in communities.
Databáze:	MEDLINE
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