| Autor: |
Dolgushin KV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia., Petrova NV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia., Iudinkova ES; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia., Razin SV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.; Biology Department, Moscow State University, Moscow, 119991 Russia., Iarovaia OV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.; iarovaia@inbox.ru. |
| Jazyk: |
ruština |
| Zdroj: |
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2015 May-Jun; Vol. 49 (3), pp. 498-506. |
| DOI: |
10.7868/S0026898415030040 |
| Abstrakt: |
In the domain model of eukaryotic genome organization, the functional unit of the genome, along with the relevant regulatory elements, is considered to be a gene or a gene family. In hot-blooded vertebrate animals, the domains of a- and b-globin genes are positioned at different chromosomes and are organized and regulated in different fashion. In cold-blooded animals, in particular in tropical fish Danio rerio, a- and b globin genes are located in a common gene cluster. However, the joint a/b-globin gene cluster is subdivided into two development stage-specific subdomains, the adult one and the embryonic-larval one. In an attempt to find out whether this functional segregation correlates with structural segregation of the domain we compared the DNase I sensitivity and profiles of histone modifications of adult and embryonic-larval segments of the domain in cultured D. rerio fibroblasts. We have demonstrated that, in these nonerythroid cells, adult and embryonic- larval subdomains possess different DNase I sensitivities and different profiles of H3K27me3, a histone modification introduced by PRC2 complex. These observations suggest that joint a/b globin gene domain of Danio rerio is segregated into two structural subdomain harboring adult and embryonic-larval globin genes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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