Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis.

Autor:	Leão SC; Universidade Federal de São Paulo, São Paulo, SP, Brazil., Dashwood MR; Department of Clinical Biochemistry, Medical School, University College London, London, UK., Andrade MS; Group of Molecular Anatomy, Universidade Federal de Sergipe, São Cristóvão, SE, Brazil., Santos NN; Group of Molecular Anatomy, Universidade Federal de Sergipe, São Cristóvão, SE, Brazil., Teles OR; Group of Molecular Anatomy, Universidade Federal de Sergipe, São Cristóvão, SE, Brazil., Souza WB; Group of Molecular Anatomy, Universidade Federal de Sergipe, São Cristóvão, SE, Brazil., Rodrigues TM; Group of Molecular Anatomy, Universidade Federal de Sergipe, São Cristóvão, SE, Brazil.
Jazyk:	angličtina
Zdroj:	Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular [Rev Bras Cir Cardiovasc] 2015 Mar-Apr; Vol. 30 (2), pp. 211-8.
DOI:	10.5935/1678-9741.20150004
Abstrakt:	Introduction: Rheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A. Objective: The aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves. Methods: Immunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples. Results: ET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21 ± 14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06 ± 13.13% and 9.20 ± 11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R = 0.74, P = 0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R = -0.37, P = 0.25), and ETB (R = -0.14, P = 0.39). This data was supported by western blot analysis. Conclusion: The strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.
Databáze:	MEDLINE
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