Investigation of irradiated rats DNA in the presence of Cu(II) chelates of amino acids Schiff bases.

Autor:	Karapetyan NH; a Department of Physics , Yerevan State University , Al. Manoogian 1, Yerevan 0025 , Armenia., Torosyan AL; a Department of Physics , Yerevan State University , Al. Manoogian 1, Yerevan 0025 , Armenia., Malakyan M; b Centre of Radiation Medicine and Burns , P.O. Box 25, Davidashen, Yerevan 0048 , Armenia., Bajinyan SA; b Centre of Radiation Medicine and Burns , P.O. Box 25, Davidashen, Yerevan 0048 , Armenia., Haroutiunian SG; a Department of Physics , Yerevan State University , Al. Manoogian 1, Yerevan 0025 , Armenia.
Jazyk:	angličtina
Zdroj:	Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 2016; Vol. 34 (1), pp. 177-83. Date of Electronic Publication: 2015 Jun 23.
DOI:	10.1080/07391102.2015.1020876
Abstrakt:	The new synthesized Cu(II) chelates of amino acids Schiff bases were studied as a potential radioprotectors. Male albino rats of Wistar strain were exposed to X-ray whole-body irradiation at 4.8 Gy. This dose caused 30% mortality of the animals (LD30). The survival of animals exposed to radiation after preliminary administration of 10 mg/kg Cu(II)(Nicotinyl-L-Tyrosinate)2 or Cu(II)(Nicotinyl-L-Tryptophanate)2 prior to irradiation was registered about 80 and 100% correspondingly. Using spectrophotometric melting and agarose gel electrophoresis methods, the differences between the DNA isolated from irradiated rats and rats pretreated with Cu(II) chelates were studied. The fragments of DNA with different breaks were revealed in DNA samples isolated from irradiated animals. While, the repair of the DNA structure was observed for animals pretreated with the Cu(II) chelates. The results suggested that pretreatment of the irradiated rats with Cu(II)(Nicotinyl-L-Tyrosinate)2 and Cu(II)(Nicotinyl-L-Tryptophanate)2 compounds improves the liver DNA characteristics.
Databáze:	MEDLINE
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