A Forward Genetic Screen for Suppressors of Somatic P Granules in Caenorhabditis elegans.

Autor: Kelly AL; Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672., Senter-Zapata MJ; Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672., Campbell AC; Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672., Lust HE; Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672., Theriault ME; Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672., Andralojc KM; Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672., Updike DL; Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672 dupdike@mdibl.org.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2015 Jun 22; Vol. 5 (10), pp. 2209-15. Date of Electronic Publication: 2015 Jun 22.
DOI: 10.1534/g3.115.019257
Abstrakt: In Caenorhabditis elegans, germline expression programs are actively repressed in somatic tissue by components of the synMuv (synthetic multi-vulva) B chromatin remodeling complex, which include homologs of tumor suppressors Retinoblastoma (Rb/LIN-35) and Malignant Brain Tumor (MBT/LIN-61). However, the full scope of pathways that suppress germline expression in the soma is unknown. To address this, we performed a mutagenesis and screened for somatic expression of GFP-tagged PGL-1, a core P-granule nucleating protein. Eight alleles were isolated from 4000 haploid genomes. Five of these alleles exhibit a synMuv phenotype, whereas the remaining three were identified as hypomorphic alleles of known synMuv B genes, lin-13 and dpl-1. These findings suggest that most suppressors of germline programs in the soma of C. elegans are either required for viability or function through synMuv B chromatin regulation.
(Copyright © 2015 Kelly et al.)
Databáze: MEDLINE
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