A Versatile Phenotyping System and Analytics Platform Reveals Diverse Temporal Responses to Water Availability in Setaria.

Autor: Fahlgren N; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Feldman M; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Gehan MA; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Wilson MS; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Shyu C; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Bryant DW; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Hill ST; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., McEntee CJ; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Warnasooriya SN; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Kumar I; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Ficor T; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Turnipseed S; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Gilbert KB; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Brutnell TP; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Carrington JC; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Mockler TC; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA., Baxter I; USDA-ARS, Donald Danforth Plant Science Center, St. Louis, MO 63132, USA. Electronic address: ivan.baxter@ars.usda.gov.
Jazyk: angličtina
Zdroj: Molecular plant [Mol Plant] 2015 Oct 05; Vol. 8 (10), pp. 1520-35. Date of Electronic Publication: 2015 Jun 20.
DOI: 10.1016/j.molp.2015.06.005
Abstrakt: Phenotyping has become the rate-limiting step in using large-scale genomic data to understand and improve agricultural crops. Here, the Bellwether Phenotyping Platform for controlled-environment plant growth and automated multimodal phenotyping is described. The system has capacity for 1140 plants, which pass daily through stations to record fluorescence, near-infrared, and visible images. Plant Computer Vision (PlantCV) was developed as open-source, hardware platform-independent software for quantitative image analysis. In a 4-week experiment, wild Setaria viridis and domesticated Setaria italica had fundamentally different temporal responses to water availability. While both lines produced similar levels of biomass under limited water conditions, Setaria viridis maintained the same water-use efficiency under water replete conditions, while Setaria italica shifted to less efficient growth. Overall, the Bellwether Phenotyping Platform and PlantCV software detected significant effects of genotype and environment on height, biomass, water-use efficiency, color, plant architecture, and tissue water status traits. All ∼ 79,000 images acquired during the course of the experiment are publicly available.
(Copyright © 2015 The Author. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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