The coral immune response facilitates protection against microbes during tissue regeneration.

Autor: van de Water JA; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Qld 4811, Australia.; College of Marine and Environmental Sciences, James Cook University, Townsville, Qld 4811, Australia.; AIMS@JCU, James Cook University, Townsville, Qld 4811, Australia.; Australian Institute of Marine Science, PMB 3, Townsville MC, Townsville, Qld 4810, Australia., Ainsworth TD; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Qld 4811, Australia., Leggat W; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Qld 4811, Australia.; College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Qld 4811, Australia., Bourne DG; AIMS@JCU, James Cook University, Townsville, Qld 4811, Australia.; Australian Institute of Marine Science, PMB 3, Townsville MC, Townsville, Qld 4810, Australia., Willis BL; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Qld 4811, Australia.; College of Marine and Environmental Sciences, James Cook University, Townsville, Qld 4811, Australia.; Australian Institute of Marine Science, PMB 3, Townsville MC, Townsville, Qld 4810, Australia., van Oppen MJ; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Qld 4811, Australia.; AIMS@JCU, James Cook University, Townsville, Qld 4811, Australia.; Australian Institute of Marine Science, PMB 3, Townsville MC, Townsville, Qld 4810, Australia.
Jazyk: angličtina
Zdroj: Molecular ecology [Mol Ecol] 2015 Jul; Vol. 24 (13), pp. 3390-404. Date of Electronic Publication: 2015 Jun 19.
DOI: 10.1111/mec.13257
Abstrakt: Increasing physical damage on coral reefs from predation, storms and anthropogenic disturbances highlights the need to understand the impact of injury on the coral immune system. In this study, we examined the regulation of the coral immune response over 10 days following physical trauma artificially inflicted on in situ colonies of the coral Acropora aspera, simultaneously with bacterial colonization of the lesions. Corals responded to injury by increasing the expression of immune system-related genes involved in the Toll-like and NOD-like receptor signalling pathways and the lectin-complement system in three phases (<2, 4 and 10 days post-injury). Phenoloxidase activity was also significantly upregulated in two phases (<3 and 10 days post-injury), as were levels of non-fluorescent chromoprotein. In addition, green fluorescent protein expression was upregulated in response to injury from 4 days post-injury, while cyan fluorescent protein expression was reduced. No shifts in the composition of coral-associated bacterial communities were evident following injury based on 16S rRNA gene amplicon pyrosequencing. Bacteria-specific fluorescence in situ hybridization also showed no evidence of bacterial colonization of the wound or regenerating tissues. Coral tissues showed near-complete regeneration of lesions within 10 days. This study demonstrates that corals exhibit immune responses that support rapid recovery following physical injury, maintain coral microbial homeostasis and prevent bacterial infestation that may compromise coral fitness.
(© 2015 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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