The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle.

Autor: Nixon FM; Division of Biomedical Cell Biology, Warwick Medical School, Coventry, United Kingdom., Gutiérrez-Caballero C; Division of Biomedical Cell Biology, Warwick Medical School, Coventry, United Kingdom., Hood FE; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom., Booth DG; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom., Prior IA; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom., Royle SJ; Division of Biomedical Cell Biology, Warwick Medical School, Coventry, United Kingdom.
Jazyk: angličtina
Zdroj: ELife [Elife] 2015 Jun 19; Vol. 4. Date of Electronic Publication: 2015 Jun 19.
DOI: 10.7554/eLife.07635
Abstrakt: Kinetochore fibers (K-fibers) of the mitotic spindle are force-generating units that power chromosome movement during mitosis. K-fibers are composed of many microtubules that are held together throughout their length. Here, we show, using 3D electron microscopy, that K-fiber microtubules (MTs) are connected by a network of MT connectors. We term this network 'the mesh'. The K-fiber mesh is made of linked multipolar connectors. Each connector has up to four struts, so that a single connector can link up to four MTs. Molecular manipulation of the mesh by overexpression of TACC3 causes disorganization of the K-fiber MTs. Optimal stabilization of K-fibers by the mesh is required for normal progression through mitosis. We propose that the mesh stabilizes K-fibers by pulling MTs together and thereby maintaining the integrity of the fiber. Our work thus identifies the K-fiber meshwork of linked multipolar connectors as a key integrator and determinant of K-fiber structure and function.
Databáze: MEDLINE
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