Altered Activity and Expression of Cytosolic Peptidases in Colorectal Cancer.

Autor:	Perez I; 1. Department of Nursing I, School of Nursing, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., Blanco L; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., Sanz B; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., Errarte P; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., Ariz U; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., Beitia M; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., Fernández A; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., Loizate A; 3. Department of Surgery, Basurto University Hospital, University of the Basque Country (UPV/EHU), Bilbao, Bizkaia, Spain., Candenas ML; 4. Institute for Chemical Research, CSIC-Isla de la Cartuja, Sevilla, Spain;, Pinto FM; 4. Institute for Chemical Research, CSIC-Isla de la Cartuja, Sevilla, Spain;, Gil J; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., López JI; 5. Department of Anatomic Pathology, Cruces University Hospital, University of the Basque Country (UPV/EHU), Barakaldo, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain., Larrinaga G; 1. Department of Nursing I, School of Nursing, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 2. Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain ; 6. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain.
Jazyk:	angličtina
Zdroj:	International journal of medical sciences [Int J Med Sci] 2015 Jun 02; Vol. 12 (6), pp. 458-67. Date of Electronic Publication: 2015 Jun 02 (Print Publication: 2015).
DOI:	10.7150/ijms.11808
Abstrakt:	Background and Objective: The role of peptidases in carcinogenic processes and their potential usefulness as tumor markers in colorectal cancer (CRC) have been classically attributed to cell-surface enzymes. The objective of the present study was to analyze the activity and mRNA expression of three cytosolic peptidases in the CRC and to correlate the obtained results with classic histopathological parameters for tumor prognosis and survival. Methods: The activity and mRNA levels of puromycin-sensitive aminopeptidase (PSA), aminopeptidase B (APB) and pyroglutamyl-peptidase I (PGI) were measured by fluorimetric and quantitative RT-PCR methods in colorectal mucosa and tumor tissues and plasma samples from CRC patients (n=81). Results: 1) PSA and APB activity was higher in adenomas and carcinomas than in the uninvolved mucosa. 2) mRNA levels of PSA and PGI was lower in tumors. 3) PGI activity in CRC tissue correlated negatively with histological grade, tumor size and 5-year overall survival of CRC patients. 4) Higher plasmatic APB activity was independently associated with better 5-year overall survival. Conclusions: Data suggest that cytosolic peptidases may be involved in colorectal carcinogenesis and point to the determination of this enzymes as a valuable method in the determination of CRC prognosis.
Databáze:	MEDLINE
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