Role of N-WASP in Endothelial Monolayer Formation and Integrity.

Autor:	Mooren OL; From the Department of Cell Biology and Physiology, Washington University, St. Louis, Missouri 63110., Kim J; From the Department of Cell Biology and Physiology, Washington University, St. Louis, Missouri 63110., Li J; From the Department of Cell Biology and Physiology, Washington University, St. Louis, Missouri 63110., Cooper JA; From the Department of Cell Biology and Physiology, Washington University, St. Louis, Missouri 63110 jacooper@wustl.edu.
Jazyk:	angličtina
Zdroj:	The Journal of biological chemistry [J Biol Chem] 2015 Jul 24; Vol. 290 (30), pp. 18796-805. Date of Electronic Publication: 2015 Jun 12.
DOI:	10.1074/jbc.M115.668285
Abstrakt:	Endothelial cells (ECs) form a monolayer that serves as a barrier between the blood and the underlying tissue. ECs tightly regulate their cell-cell junctions, controlling the passage of soluble materials and immune cells across the monolayer barrier. We studied the role of N-WASP, a key regulator of Arp2/3 complex and actin assembly, in EC monolayers. We report that N-WASP regulates endothelial monolayer integrity by affecting the organization of cell junctions. Depletion of N-WASP resulted in an increase in transendothelial electrical resistance, a measure of monolayer integrity. N-WASP depletion increased the width of cell-cell junctions and altered the organization of F-actin and VE-cadherin at junctions. N-WASP was not present at cell-cell junctions in monolayers under resting conditions, but it was recruited following treatment with sphingosine-1-phosphate. Taken together, our results reveal a novel role for N-WASP in remodeling EC junctions, which is critical for monolayer integrity and function. (© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.)
Databáze:	MEDLINE
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