Direct detection and quantification of malondialdehyde vapour in humid air using selected ion flow tube mass spectrometry supported by gas chromatography/mass spectrometry.

Autor: Shestivska V; J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Dolejškova 3, 18223, Prague 8, Czech Republic., Antonowicz SS; Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, Praed Street, London, W2 1NY, UK., Dryahina K; J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Dolejškova 3, 18223, Prague 8, Czech Republic., Kubišta J; J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Dolejškova 3, 18223, Prague 8, Czech Republic., Smith D; Institute for Science and Technology in Medicine, School of Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK., Španěl P; J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Dolejškova 3, 18223, Prague 8, Czech Republic.; Institute for Science and Technology in Medicine, School of Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK.
Jazyk: angličtina
Zdroj: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2015 Jun 15; Vol. 29 (11), pp. 1069-79.
DOI: 10.1002/rcm.7198
Abstrakt: Rationale: It has been proposed that malondialdehyde (MDA) reflects free oxygen-radical lipid peroxidation and can be useful as a biomarker to track this process. For the analysis of MDA molecules in humid air by selected ion flow tube mass spectrometry (SIFT-MS), the rate coefficients and the ion product distributions for the reactions of the SIFT-MS reagent ions with volatile MDA in the presence of water vapour are required.
Methods: The SIFT technique has been used to determine the rate coefficients and ion product distributions for the reactions of H3O(+), NO(+) and O2 (+•) with gas-phase MDA. In support of the SIFT-MS analysis of MDA, solid-phase microextraction, SPME, coupled with gas chromatography/mass spectrometry, GC/MS, has been used to confirm the identification of MDA.
Results: The primary product ions have been identified for the reactions of H3O(+), NO(+) and O2 (+•) with MDA and the formation of their hydrates formed in humid samples is described. The following combinations of reagent and the analyte ions (given as m/z values) have been adopted for SIFT-MS analyses of MDA in the gas phase: H3O(+): 109; NO(+): 89, 102; O2 (+•): 72, 90, 108, 126. The detection and quantification of MDA released by a cell culture by SIFT-MS are demonstrated.
Conclusions: This detailed study has provided the kinetics data required for the SIFT-MS analysis of MDA in humid air, including exhaled breath and the headspace of liquid-phase biogenic media. The detection and quantification by SIFT-MS of MDA released by a cell culture are demonstrated.
(Copyright © 2015 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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