Specific humoral response of hosts with variable schistosomiasis susceptibility.

Autor: Driguez P; Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia., McWilliam HE; Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia., Gaze S; Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, Queensland Tropical Health Alliance Laboratory, James Cook University, Cairns, Queensland, Australia.; Centro de Pesquisas René Rachou, Oswaldo Cruz Foundation, Belo Horizonte, Brazil., Piedrafita D; School of Applied Sciences and Engineering, Federation University, Churchill, Victoria, Australia., Pearson MS; Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, Queensland Tropical Health Alliance Laboratory, James Cook University, Cairns, Queensland, Australia., Nakajima R; Department of Medicine, Division of Infectious Diseases, University of California Irvine, Irvine, CA, USA., Duke M; Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia., Trieu A; Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia., Doolan DL; Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia., Cardoso FC; Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.; Institute for Molecular Biosciences, University of Queensland, St Lucia, Queensland, Australia., Jasinskas A; Department of Medicine, Division of Infectious Diseases, University of California Irvine, Irvine, CA, USA., Gobert GN; Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia., Felgner PL; Department of Medicine, Division of Infectious Diseases, University of California Irvine, Irvine, CA, USA., Loukas A; Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, Queensland Tropical Health Alliance Laboratory, James Cook University, Cairns, Queensland, Australia., Meeusen E; Department of Microbiology, School of Biomedical Sciences, Monash University, Melbourne, Victoria, Australia., McManus DP; Infectious Disease Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Jazyk: angličtina
Zdroj: Immunology and cell biology [Immunol Cell Biol] 2016 Jan; Vol. 94 (1), pp. 52-65. Date of Electronic Publication: 2015 Jun 05.
DOI: 10.1038/icb.2015.61
Abstrakt: The schistosome blood flukes are some of the largest global causes of parasitic morbidity. Further study of the specific antibody response during schistosomiasis may yield the vaccines and diagnostics needed to combat this disease. Therefore, for the purposes of antigen discovery, sera and antibody-secreting cell (ASC) probes from semi-permissive rats and sera from susceptible mice were used to screen a schistosome protein microarray. Following Schistosoma japonicum infection, rats had reduced pathology, increased antibody responses and broader antigen recognition profiles compared with mice. With successive infections, rat global serological reactivity and the number of recognized antigens increased. The local antibody response in rat skin and lung, measured with ASC probes, increased after parasite migration and contributed antigen-specific antibodies to the multivalent serological response. In addition, the temporal variation of anti-parasite serum antibodies after infection and reinfection followed patterns that appear related to the antigen driving the response. Among the 29 antigens differentially recognized by the infected hosts were numerous known vaccine candidates, drug targets and several S. japonicum homologs of human schistosomiasis resistance markers-the tegument allergen-like proteins. From this set, we prioritized eight proteins that may prove to be novel schistosome vaccine and diagnostic antigens.
Databáze: MEDLINE
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