Determination of the total content of some sulfonamides in milk using solid-phase extraction coupled with off-line derivatization and spectrophotometric detection.

Autor: Dmitrienko SG; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia., Kochuk EV; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia., Tolmacheva VV; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia., Apyari VV; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia. Electronic address: apyari@mail.ru., Zolotov YA; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow 119991, Russia.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2015 Dec 01; Vol. 188, pp. 51-6. Date of Electronic Publication: 2015 Apr 27.
DOI: 10.1016/j.foodchem.2015.04.123
Abstrakt: A simple screening method for isolation and determination of the total content of some sulfonamides in milk using solid-phase extraction and a color reaction is described. This procedure is based on SPE of sulfonamides on hypercrosslinked polystyrene, elution with acetonitrile and off-line derivatization with p-dimethylaminocinnamaldehyde in acetonitrile followed by spectrophotometric determination. The reaction produces intense violet-red color and can be easily used both for quantitation of sulfonamides using spectrophotometry and for naked-eye semi-quantitative estimation. Maximum absorption of the reaction product was determined at 540 nm. The Lambert-Beer's law was obeyed in the range of 0.07-3.0 μg mL(-1) in eluate, with the squared correlation coefficient (R(2)) of 0.9875-0.9995, and the relative standard deviation (RSD) of 3-4%. The limits of SAs detection using preconcentration were of 0.02-0.03 μg mL(-1). The proposed method can be recommended as a routine screening method for quantitation of sulfonamides in milk.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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