A novel peptide-based recognition probe for the sensitive detection of CD44 on breast cancer stem cells.

Autor: Cho JH; Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea., Lee SC; Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea., Ha NR; Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea., Lee SJ; Department of Life Science, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea., Yoon MY; Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea. Electronic address: myyoon@hanyang.ac.kr.
Jazyk: angličtina
Zdroj: Molecular and cellular probes [Mol Cell Probes] 2015 Dec; Vol. 29 (6), pp. 492-499. Date of Electronic Publication: 2015 May 30.
DOI: 10.1016/j.mcp.2015.05.014
Abstrakt: Metastasis and recurrence of breast cancer remain significant clinical problems. The expression level of CD44 protein is higher in breast cancer-initiating cancer stem cells; therefore, the early detection of CD44 using a sensitive diagnostic probe is important for breast cancer diagnosis and therapeutic purposes. In this study, we fabricated a polyvalent directed peptide polymer (PDPP) that specifically recognized the CD44 biomarker, as confirmed by immunocytochemistry tests and fluorescence-activated cell sorting assessment. Our results indicate that PDPP is useful as a novel tool for the sensitive detection of breast cancer stem cells.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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