[The value of blastocyst culture on preimplantation genetic diagnosis].

Autor:	Ou J; Center of Reproduction and Genetics, Suzhou Municipal Hospital, Suzhou, Jiangsu 215002, P.R.China. hongliszivf@163.com; richardchoy@cuhk.edu.hk., Wang W, Ma Y, Zhou Z, Ding J, Wang F, Duan C, Li L, Zheng A, Chong W, Choy R, Li H
Jazyk:	čínština
Zdroj:	Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics [Zhonghua Yi Xue Yi Chuan Xue Za Zhi] 2015 Jun; Vol. 32 (3), pp. 312-7.
DOI:	10.3760/cma.j.issn.1003-9406.2015.03.002
Abstrakt:	Objective: To estimate the value of blastocyst culture for preimplantation genetic diagnosis (PGD). Methods: Day 3 embryos were biopsied and analyzed with fluorescence in situ hybridization (FISH) technique. Embryos with normal FISH results were cultured into blastocysts, and the ones with better morphology scores were transferred. Fourteen embryos with abnormal FISH results were cultured into blastocysts. Part of the cells taken from the blastocysts were amplified by whole genomic amplification (WGA) and assessed by array-based comparative genomic hybridization (array-CGH) analysis. Results: Six blastocysts with normal FISH results were transferred in 5 cycles. Four healthy babies of 3 cycles were delivered. Another one was a singleton pregnancy but with embryo growth arrest, whose villus karyotype was normal. Fourteen embryos with abnormal FISH results were cultured into blastocysts and analyzed by array-CGH. Six blastocysts were normal by array-CGH. Conclusion: FISH combined with blastocyst culture may further ensure the accuracy of PGD result. Detection at the blastocyst stage can avoid false positive results and mosaic interferences on Day 3 stage and are therefore more authentic.
Databáze:	MEDLINE
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