Production of oleanane-type sapogenin in transgenic rice via expression of β-amyrin synthase gene from Panax japonicus C. A. Mey.
| Autor: | Huang Z; College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. hzwfau@163.com.; Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. hzwfau@163.com., Lin J; Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. linjc2011@126.com., Cheng Z; Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. chengzuxin@163.com., Xu M; Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. xmfau@163.com., Guo M; Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. 115942082@qq.com., Huang X; Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. 764900262@qq.com., Yang Z; Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. yangzj41@163.com., Zheng J; Agricultural Product Quality Institute, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, CangShan District, Fuzhou, 350002, Fujian, China. zjg231@126.com. |
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| Jazyk: | angličtina |
| Zdroj: | BMC biotechnology [BMC Biotechnol] 2015 Jun 02; Vol. 15, pp. 45. Date of Electronic Publication: 2015 Jun 02. |
| DOI: | 10.1186/s12896-015-0166-4 |
| Abstrakt: | Background: Panax japonicus C. A. Mey. is a rare traditional Chinese herbal medicine that uses ginsenosides as its main active ingredient. Rice does not produce ginsenosides because it lacks a key rate-limiting enzyme (β-amyrin synthase, βAS); however, it produces a secondary metabolite, 2,3-oxidosqualene, which is a precursor for ginsenoside biosynthesis. Results: In the present study, the P. japonicus βAS gene was transformed into the rice cultivar 'Taijing 9' using an Agrobacterium-mediated approach, resulting in 68 rice transgenic plants of the T0 generation. Transfer-DNA (T-DNA) insertion sites in homozygous lines of the T2 generation were determined by using high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR) and were found to vary among the tested lines. Approximately 1-2 copies of the βAS gene were detected in transgenic rice plants. Real-time PCR and Western blotting analyses showed that the transformed βAS gene could be overexpressed and β-amyrin synthase could be expressed in rice. HPLC analysis showed that the concentration of oleanane-type sapogenin oleanolic acid in transgenic rice was 8.3-11.5 mg/100 g dw. Conclusions: The current study is the first report on the transformation of P. japonicus βAS gene into rice. We have successfully produced a new rice germplasm, "ginseng rice", which produces oleanane-type sapogenin. |
| Databáze: | MEDLINE |
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