Periapical Lesions Decrease Insulin Signaling in Rat Skeletal Muscle.

Autor:	Astolphi RD; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil., Curbete MM; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil., Chiba FY; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil., Cintra LT; Department of Endodontics, Araçatuba Dental School, Araçatuba, São Paulo, Brazil., Ervolino E; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil., da Mota MS; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil., Antoniali C; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil., Garbin CA; Department of Child and Social Dentistry, Araçatuba Dental School, UNESP, Araçatuba, São Paulo, Brazil., Sumida DH; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: dorishs@foa.unesp.br.
Jazyk:	angličtina
Zdroj:	Journal of endodontics [J Endod] 2015 Aug; Vol. 41 (8), pp. 1305-10. Date of Electronic Publication: 2015 May 28.
DOI:	10.1016/j.joen.2015.04.002
Abstrakt:	Introduction: Serum inflammatory cytokines derived from oral inflammation are associated with decreased insulin signaling (IS) and insulin resistance, which is a major risk factor for type 2 diabetes mellitus. This study aimed to investigate IS in the liver and skeletal muscle (SM) and disorders related to the serum lipid profile and glucose and insulin levels of nondiabetic rats with induced chronic periapical lesions (PLs). Methods: Twenty-eight Wistar rats were divided into control and PL groups. PLs were induced by exposing the pulpal tissue to the oral environment. Experiments were conducted in both groups 30 days after pulp exposure. Maxillae were processed for histopathological analysis. IS was evaluated according to insulin receptor substrate (pp185-insulin receptor substrate 1 [IRS-1]/insulin receptor substrate 2 [IRS-2]) tyrosine phosphorylation status, IRS-1 serine phosphorylation status, and IRS-1 and IRS-2 content in the liver and SM by Western blotting. Serum total cholesterol, triglyceride, glucose, and insulin levels were measured enzymatically using a commercial kit. Results: PL rats showed reduced pp185 P-Tyr and increased IRS-1 serine phosphorylation status in the SM but no change in the liver after insulin stimulation. No significant changes in IRS-1 and IRS-2 content, serum total cholesterol, triglyceride, glucose or insulin levels were noted. Conclusions: PLs are associated with decreased insulin signaling in the SM of rats. Because a decrease in insulin signaling is associated with insulin resistance, our results emphasize the importance of preventing local inflammatory diseases such as PLs to prevent alterations in IS in muscle. (Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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