| Autor: |
Wieckhusen C; Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Service Baden-Württemberg - Hessen, Friedrich-Ebert-Straße 107, Mannheim, 68167, Germany, wieckhusen@stud.uni-heidelberg.de., Bugert P |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1310, pp. 123-33. |
| DOI: |
10.1007/978-1-4939-2690-9_11 |
| Abstrakt: |
The KEL (ISBT 006), JR (ISBT 032), and LAN (ISBT 033) blood group systems are defined by a complex genetics with a large number of exons, and numerous gene variants. In order to sequence the 53 coding exons and flanking intron regions of all three blood group genes we developed a next-generation-sequencing method using 454-sequencing™ on a Genome Sequencer (GS) Junior™ system. Multiplex analysis of eight individual DNA samples was achieved using molecular identifiers. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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