Expression profile of microRNAs in gastrointestinal stromal tumors revealed by high throughput quantitative RT-PCR microarray.

Autor:	Tong HX; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Zhou YH; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Hou YY; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Zhang Y; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Huang Y; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Xie B; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Wang JY; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Jiang Q; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., He JY; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Shao YB; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Han WM; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Tan RY; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Zhu J; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China., Lu WQ; Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2015 May 21; Vol. 21 (19), pp. 5843-55.
DOI:	10.3748/wjg.v21.i19.5843
Abstrakt:	Aim: To investigate the microRNA (miRNA) expression profile in gastrointestinal stromal tumor (GIST) tissues that could serve as a novel diagnostic biomarker for GIST detection. Methods: We performed a quantitative real-time quantitative reverse transcriptase polymerase chain reaction assay to analyze the expression of 1888 miRNAs in a sample set that included 54 GIST tissue samples. Results: We found that dysregulation of several miRNAs may be related to the malignant potential of GISTs. Six of these miRNAs, hsa-let-7c, miR-218, miR-488#, miR-4683, miR-34c-5p and miR-4773, were selected as the final list of biomarkers to separate the malignant GISTs (M group) from the benign GISTs (B group). In addition, MiR-29b-2#, hsa-let-7c, miR-891b, miR-218, miR-204, miR-204-3p, miR-628-5p, miR-744, miR-29c#, miR-625 and miR-196a were used to distinguish between the borderline (BO group) and M groups. There were 11 common miRNAs selected to separate the benign and borderline (BB) group from the M group, including hsa-let-7c, miR-218, miR-628-5p, miR-204-3p, miR-204, miR-891b, miR-488#, miR-145, miR-891a, miR-34c-5p and miR-196a. Conclusion: The identified miRNAs appear to be novel biomarkers to distinguish malignant from benign GISTs, which may be helpful to understand the mechanisms of GIST oncogenesis and progression, and to further elucidate the characteristics of GIST subtypes.
Databáze:	MEDLINE
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