Complete Genome Sequence and Transcriptomic Analysis of the Novel Pathogen Elizabethkingia anophelis in Response to Oxidative Stress.

Autor:	Li Y; School of Biological Sciences, Nanyang Technological University, Singapore., Liu Y; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore., Chew SC; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore Interdisciplinary Graduate School, Nanyang Technological University, Singapore., Tay M; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore., Salido MM; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore., Teo J; Microbiology Unit, Department of Laboratory Medicine, National University Hospital, Singapore., Lauro FM; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia., Givskov M; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, Denmark., Yang L; School of Biological Sciences, Nanyang Technological University, Singapore Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore yangliang@ntu.edu.sg.
Jazyk:	angličtina
Zdroj:	Genome biology and evolution [Genome Biol Evol] 2015 May 26; Vol. 7 (6), pp. 1676-85. Date of Electronic Publication: 2015 May 26.
DOI:	10.1093/gbe/evv101
Abstrakt:	Elizabethkingia anophelis is an emerging pathogen that can cause life-threatening infections in neonates, severely immunocompromised and postoperative patients. The lack of genomic information on E. anophelis hinders our understanding of its mechanisms of pathogenesis. Here, we report the first complete genome sequence of E. anophelis NUHP1 and assess its response to oxidative stress. Elizabethkingia anophelis NUHP1 has a circular genome of 4,369,828 base pairs and 4,141 predicted coding sequences. Sequence analysis indicates that E. anophelis has well-developed systems for scavenging iron and stress response. Many putative virulence factors and antibiotic resistance genes were identified, underscoring potential host-pathogen interactions and antibiotic resistance. RNA-sequencing-based transcriptome profiling indicates that expressions of genes involved in synthesis of an yersiniabactin-like iron siderophore and heme utilization are highly induced as a protective mechanism toward oxidative stress caused by hydrogen peroxide treatment. Chrome azurol sulfonate assay verified that siderophore production of E. anophelis is increased in the presence of oxidative stress. We further showed that hemoglobin facilitates the growth, hydrogen peroxide tolerance, cell attachment, and biofilm formation of E. anophelis NUHP1. Our study suggests that siderophore production and heme uptake pathways might play essential roles in stress response and virulence of the emerging pathogen E. anophelis. (© The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)
Databáze:	MEDLINE
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