Comparative proteomics analysis of the antitumor effect of CIGB-552 peptide in HT-29 colon adenocarcinoma cells.

Autor: Núñez de Villavicencio-Díaz T; Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba., Ramos Gómez Y; Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba., Oliva Argüelles B; Pharmaceuticals Department, Center for Genetic Engineering and Biotechnology, Cuba., Fernández Masso JR; Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba., Rodríguez-Ulloa A; Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba., Cruz García Y; Department of Preclinical Studies, National Institute of Oncology and Radiobiology of Cuba, Cuba., Guirola-Cruz O; Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba., Perez-Riverol Y; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, UK., Javier González L; Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba., Tiscornia I; Cell Biology Unit, Institut Pasteur of Montevideo, Uruguay., Victoria S; Cell Biology Unit, Institut Pasteur of Montevideo, Uruguay., Bollati-Fogolín M; Cell Biology Unit, Institut Pasteur of Montevideo, Uruguay., Besada Pérez V; Department of Systems Biology, Center for Genetic Engineering and Biotechnology, Cuba. Electronic address: vladimir.besada@cigb.edu.cu., Guerra Vallespi M; Pharmaceuticals Department, Center for Genetic Engineering and Biotechnology, Cuba.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2015 Aug 03; Vol. 126, pp. 163-71. Date of Electronic Publication: 2015 May 24.
DOI: 10.1016/j.jprot.2015.05.024
Abstrakt: The second generation peptide CIGB-552 has a pro-apoptotic effect on H460 non-small cell lung cancer cells and displays a potent cytotoxic effect in HT-29 colon adenocarcinoma cells though its action mechanism is ill defined. Here, we present the first proteomic study of peptide effect in HT-29 cells using subcellular fractionation, protein and peptide fractionation by DF-PAGE and LC-MS/MS peptide identification. In particular, we explored the nuclear proteome of HT-29 cells at a 5h treatment identifying a total of 68 differentially modulated proteins, 49 of which localize to the nucleus. The differentially modulated proteins were analyzed following a system biology approach. Results pointed to a modulation of apoptosis, oxidative damage removal, NF-κB activation, inflammatory signaling and of cell adhesion and motility. Further Western blot and flow-cytometry experiments confirmed both pro-apoptotic and anti-inflammatory effects of CIGB-552 peptide in HT-29 cells.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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