Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid.

Autor:	Bindman NA; †Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Champaign, Illinois 61820, United States., Bobeica SC; †Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Champaign, Illinois 61820, United States., Liu WR, van der Donk WA; †Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Champaign, Illinois 61820, United States.
Jazyk:	angličtina
Zdroj:	Journal of the American Chemical Society [J Am Chem Soc] 2015 Jun 10; Vol. 137 (22), pp. 6975-8. Date of Electronic Publication: 2015 Jun 01.
DOI:	10.1021/jacs.5b04681
Abstrakt:	The biosynthesis of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products typically involves a precursor peptide which contains a leader peptide that is important for the modification process, and that is removed in the final step by a protease. Genome mining efforts for new RiPPs are often hampered by the lack of a general method to remove the leader peptides. We describe here the incorporation of hydroxy acids into the precursor peptides in E. coli which results in connection of the leader peptide via an ester linkage that is readily cleaved by simple hydrolysis. We demonstrate the method for two lantibiotics, lacticin 481 and nukacin ISK-1.
Databáze:	MEDLINE
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