A cascade of arabinosyltransferases controls shoot meristem size in tomato.

Autor: Xu C; Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA., Liberatore KL; 1] Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA. [2] Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA., MacAlister CA; Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA., Huang Z; Department of Horticulture and Crop Science, Ohio State University, Wooster, Ohio, USA., Chu YH; Department of Horticulture and Crop Science, Ohio State University, Wooster, Ohio, USA., Jiang K; Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA., Brooks C; Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA., Ogawa-Ohnishi M; Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan., Xiong G; Energy Biosciences Institute, University of California, Berkeley, Berkeley, California, USA., Pauly M; 1] Energy Biosciences Institute, University of California, Berkeley, Berkeley, California, USA. [2] Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California, USA., Van Eck J; Boyce Thompson Institute for Plant Science, Ithaca, New York, USA., Matsubayashi Y; Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan., van der Knaap E; Department of Horticulture and Crop Science, Ohio State University, Wooster, Ohio, USA., Lippman ZB; 1] Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA. [2] Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
Jazyk: angličtina
Zdroj: Nature genetics [Nat Genet] 2015 Jul; Vol. 47 (7), pp. 784-92. Date of Electronic Publication: 2015 May 25.
DOI: 10.1038/ng.3309
Abstrakt: Shoot meristems of plants are composed of stem cells that are continuously replenished through a classical feedback circuit involving the homeobox WUSCHEL (WUS) gene and the CLAVATA (CLV) gene signaling pathway. In CLV signaling, the CLV1 receptor complex is bound by CLV3, a secreted peptide modified with sugars. However, the pathway responsible for modifying CLV3 and its relevance for CLV signaling are unknown. Here we show that tomato inflorescence branching mutants with extra flower and fruit organs due to enlarged meristems are defective in arabinosyltransferase genes. The most extreme mutant is disrupted in a hydroxyproline O-arabinosyltransferase and can be rescued with arabinosylated CLV3. Weaker mutants are defective in arabinosyltransferases that extend arabinose chains, indicating that CLV3 must be fully arabinosylated to maintain meristem size. Finally, we show that a mutation in CLV3 increased fruit size during domestication. Our findings uncover a new layer of complexity in the control of plant stem cell proliferation.
Databáze: MEDLINE
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