HPLC-UV method validation for the identification and quantification of bioactive amines in commercial eggs.

Autor: de Figueiredo TC; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil., de Assis DC; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil., Menezes LD; Instituto Mineiro de Agropecuária (IMA), Rodovia Américo Gianetti, 4001, Belo Horizonte 31.630-901, Brazil., da Silva GR; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil., Lanza IP; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil., Heneine LG; Laboratório de Imunologia Aplicada, Fundação Ezequiel Dias (FUNED), Rua Conde Pereira Carneiro, 80, Belo Horizonte 30.510-010, Brazil., Cançado Sde V; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte 30.123-970, Brazil. Electronic address: silvanavc@ufmg.br.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2015 Sep 01; Vol. 142, pp. 240-5. Date of Electronic Publication: 2015 Apr 27.
DOI: 10.1016/j.talanta.2015.04.056
Abstrakt: A quantitative and confirmatory high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method for the determination of bioactive amines in the albumen and yolk of commercial eggs was developed, optimized and validated by analyte extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine standards were used to evaluate the following performance parameters: limit of detection (LoD), limit of quantification (LoQ), selectivity, linearity, precision, recovery and ruggedness. The LoD of the method was defined from 0.2 to 0.3 mg kg(-1) for the yolk matrix and from 0.2 to 0.4 mg kg(-1) for the albumen matrix; the LoQ was from 0.7 to 1.0 mg kg(-1) for the yolk matrix and from 0.7 to 1.1 mg kg(-1) for the albumen matrix. The validated method exhibited excellent selectivity and separation of all amines with coefficients of determination higher than 0.99. The obtained recovery values were from 90.5% to 108.3%, and the relative standard deviation (RSD) was lower than 10% under repeatability conditions for the studied analytes. The performance parameters show the validated method to be adequate for the determination of bioactive amines in egg albumen and yolk.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: