Fungal demethylation of Kraft lignin.

Autor: Zou L; Qinghai Academy of Agriculture and Forestry Sciences, 253 Ningda Rd, Xining, Qinghai 810016, PR China. Electronic address: zly198300@163.com., Ross BM; Northern Ontario School of Medicine, Lakehead University, Thunder Bay, Ontario, Canada P7B 5E1. Electronic address: bross@nosm.ca., Hutchison LJ; Faculty of Natural Resources Management, Lakehead University, 955 Oliver Rd, Thunder Bay, Ontario, Canada P7B 5E1. Electronic address: ljhutchi@lakeheadu.ca., Christopher LP; Biorefining Research Institute, Lakehead University, 955 Oliver Rd, Thunder Bay, Ontario, Canada P7B 5E1. Electronic address: lchristo@lakeheadu.ca., Dekker RF; Biorefining Research Institute, Lakehead University, 955 Oliver Rd, Thunder Bay, Ontario, Canada P7B 5E1., Malek L; Biorefining Research Institute, Lakehead University, 955 Oliver Rd, Thunder Bay, Ontario, Canada P7B 5E1; Department of Biology, Lakehead University, 955 Oliver Rd, Thunder Bay, Ontario, Canada P7B 5E1. Electronic address: lmalek@lakeheadu.ca.
Jazyk: angličtina
Zdroj: Enzyme and microbial technology [Enzyme Microb Technol] 2015 Jun; Vol. 73-74, pp. 44-50. Date of Electronic Publication: 2015 Apr 13.
DOI: 10.1016/j.enzmictec.2015.04.001
Abstrakt: Demethylation of industrial lignin has been for long coveted as a pathway to the production of an abundant natural substitute for fossil-oil derived phenol. In an attempt to possibly identify a novel Kraft lignin-demethylating enzyme, we surveyed a collection of fungi by using selected ion flow tube-mass spectrometry (SIFT-MS). This method readily identifies methanol resulting from lignin demethylation activity. Absidia cylindrospora, and unidentified Cylindrocladium sp. and Aspergillus sp. were shown to metabolize lignin via different pathways, based on the HPLC analysis of lignin fragments. Of these three, Cylindrocladium and Aspergillus were shown to retain most of the lignin intact after 3 weeks in culture, while removing about 40% of the available methoxy groups. Our results demonstrate that after optimization of culture and lignin recovery methods, biological modification of Kraft lignin may be a feasible pathway to obtaining demethylated lignin for further industrial use.
(Crown Copyright © 2015. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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