Orthogonally oriented scaffolds with aligned fibers for engineering intestinal smooth muscle.

Autor: Kobayashi M; Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA., Lei NY; Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA 90095, USA., Wang Q; Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA., Wu BM; Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Division of Advanced Prosthodontics & Weintraub Center for Reconstructive Biotechnology, University of California, Los Angeles, Los Angeles, CA, USA., Dunn JC; Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: jdunn@mednet.ucla.edu.
Jazyk: angličtina
Zdroj: Biomaterials [Biomaterials] 2015 Aug; Vol. 61, pp. 75-84. Date of Electronic Publication: 2015 May 15.
DOI: 10.1016/j.biomaterials.2015.05.023
Abstrakt: Controlling cellular alignment is critical in engineering intestines with desired structure and function. Although previous studies have examined the directional alignment of cells on the surface (x-y plane) of parallel fibers, quantitative analysis of the cellular alignment inside implanted scaffolds with oriented fibers has not been reported. This study examined the cellular alignment in the x-z and y-z planes of scaffolds made with two layers of orthogonally oriented fibers. The cellular orientation inside implanted scaffolds was evaluated with immunofluorescence. Quantitative analysis of coherency between cell orientation and fiber direction confirmed that cells aligned along the fibers not only on the surface (x-y plane) but also inside the scaffolds (x-z & y-z planes). Our study demonstrated that two layers of orthogonally aligned scaffolds can generate the histological organization of cells similar to that of intestinal circular and longitudinal smooth muscle.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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