| Autor: |
Voorter CE; Department of Biochemistry, University of Nijmegen, The Netherlands., de Haard-Hoekman WA, Roersma ES, Meyer HE, Bloemendal H, de Jong WW |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1989 Dec 18; Vol. 259 (1), pp. 50-2. |
| DOI: |
10.1016/0014-5793(89)81491-7 |
| Abstrakt: |
Phosphate content determinations established that in alpha B-crystallin two phosphate groups can be present in vivo in bovine lenses. Comparison of tryptic digests of phosphorylated and unphosphorylated alpha B chains, revealed the location of the two phosphorylation sites in tryptic peptides T2 and T3. Thermolytic digestion and gas-phase sequencing demonstrated that Ser-19 and Ser-45 are the in vivo phosphorylation sites of bovine alpha B-crystallin. This pattern of phosphorylation differs from the previously reported in vitro obtained results. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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