A pospiviroid from symptomless portulaca plants closely related to iresine viroid 1.

Autor: Verhoeven JT; National Plant Protection Organization, National Reference Centre, P.O. Box 9102, 6700 HC Wageningen, The Netherlands. Electronic address: j.t.j.verhoeven@nvwa.nl., Roenhorst JW; National Plant Protection Organization, National Reference Centre, P.O. Box 9102, 6700 HC Wageningen, The Netherlands., Hooftman M; Naktuinbouw, P.O. Box 40, 2370 AA Roelofarendsveen, The Netherlands., Meekes ET; Naktuinbouw, P.O. Box 40, 2370 AA Roelofarendsveen, The Netherlands., Flores R; Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Valencia 46022, Spain., Serra P; Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Valencia 46022, Spain.
Jazyk: angličtina
Zdroj: Virus research [Virus Res] 2015 Jul 02; Vol. 205, pp. 22-6. Date of Electronic Publication: 2015 May 16.
DOI: 10.1016/j.virusres.2015.05.005
Abstrakt: In symptomless plants of portulaca a potential new pospiviroid was characterized. Analysis by both double and return PAGE showed the presence of a circular RNA. RT-PCR and sequencing revealed a genome of 351 nt with properties characteristic of members of the genus Pospiviroid and with highest sequence identity (circa 80%) with iresine viroid 1 (IrVd-1). The circular RNA from portulaca was shown to replicate independently in its original host, thus showing that it is indeed a viroid. Based on its molecular characteristics, it should be considered a new species. However, since no biological differences have yet been found with its closest relative IrVd-1, the viroid from portulaca does not fulfil all criteria for species demarcation of the ICTV.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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