A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate.

Autor: Li Y; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. liyy_08@163.com.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. liyy_08@163.com., Wang W; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. wangweidong0106@163.com.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. wangweidong0106@163.com., Cheng D; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. ddCzstu1990@126.com.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. ddCzstu1990@126.com., Wang T; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. wangtao900121@163.com.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. wangtao900121@163.com., Lu C; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. luconger0302@163.com.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. luconger0302@163.com., Chen J; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. chj1999@126.com.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. chj1999@126.com., Nie Z; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. wuxinzm@zstu.edu.cn.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. wuxinzm@zstu.edu.cn., Zhang W; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. zwpcc@126.com.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. zwpcc@126.com., Lv Z; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. zhengbingl@zstu.edu.cn.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. zhengbingl@zstu.edu.cn., Wu W; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. wangweidong0106@163.com., Shu J; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. shujianhong@zstu.edu.cn.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China. shujianhong@zstu.edu.cn.
Jazyk: angličtina
Zdroj: Marine drugs [Mar Drugs] 2015 May 13; Vol. 13 (5), pp. 2955-66. Date of Electronic Publication: 2015 May 13.
DOI: 10.3390/md13052955
Abstrakt: APSL (active peptide from shark liver) is a hepatic stimulator cytokine from the liver of Chiloscyllium. It can effectively protect islet cells and improve complications in mice with alloxan-induced diabetes. Here, we demonstrate that the APSL sequence is present in the N-terminus of novel TBC (Tre-2, Bub2 and Cdc16) domain family, member 15 (TBC1D15) from Chiloscyllium plagiosum. This shark TBC1D15 gene, which contains an ORF of 2088 bp, was identified from a cDNA library of regenerating shark liver. Bioinformatic analysis showed that the gene is highly homologous to TBC1D15 genes from other species. Moreover, the N-terminus of shark TBC1D15 contains a motif of unknown function (DUF3548), which encompasses the APSL fragment. Rab-GAP activity analysis showed that shark TBC1D15 is a new member of the TBC1D15 family. These results demonstrated that shark TBC1D15 possesses Rab-GAP activity using Rab7 as a substrate, which is a common property of the TBC1D15 family. The involvement of APSL at the N-terminus of TBC1D15 also demonstrates that this protein might be involved in insulin signaling and may be associated with the development of type 2 diabetes. The current findings pave the way for further functional and clinical studies of these proteins from marine sources.
Databáze: MEDLINE
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