Gene and MicroRNA Expression Responses to Exercise; Relationship with Insulin Sensitivity.

Autor: McLean CS; School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America., Mielke C; School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America., Cordova JM; School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America., Langlais PR; Mayo Clinic in Arizona, Scottsdale, Arizona, United States of America., Bowen B; School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America., Miranda D; Mayo Clinic in Arizona, Scottsdale, Arizona, United States of America., Coletta DK; School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America., Mandarino LJ; School for the Science of Health Care Delivery, Arizona State University, Tempe, Arizona, United States of America; Mayo Clinic in Arizona, Scottsdale, Arizona, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2015 May 18; Vol. 10 (5), pp. e0127089. Date of Electronic Publication: 2015 May 18 (Print Publication: 2015).
DOI: 10.1371/journal.pone.0127089
Abstrakt: Background: Healthy individuals on the lower end of the insulin sensitivity spectrum also have a reduced gene expression response to exercise for specific genes. The goal of this study was to determine the relationship between insulin sensitivity and exercise-induced gene expression in an unbiased, global manner.
Methods and Findings: Euglycemic clamps were used to measure insulin sensitivity and muscle biopsies were done at rest and 30 minutes after a single acute exercise bout in 14 healthy participants. Changes in mRNA expression were assessed using microarrays, and miRNA analysis was performed in a subset of 6 of the participants using sequencing techniques. Following exercise, 215 mRNAs were changed at the probe level (Bonferroni-corrected P<0.00000115). Pathway and Gene Ontology analysis showed enrichment in MAP kinase signaling, transcriptional regulation and DNA binding. Changes in several transcription factor mRNAs were correlated with insulin sensitivity, including MYC, r=0.71; SNF1LK, r=0.69; and ATF3, r= 0.61 (5 corrected for false discovery rate). Enrichment in the 5'-UTRs of exercise-responsive genes suggested regulation by common transcription factors, especially EGR1. miRNA species of interest that changed after exercise included miR-378, which is located in an intron of the PPARGC1B gene.
Conclusions: These results indicate that transcription factor gene expression responses to exercise depend highly on insulin sensitivity in healthy people. The overall pattern suggests a coordinated cycle by which exercise and insulin sensitivity regulate gene expression in muscle.
Databáze: MEDLINE
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