One-Step Formation of "Chain-Armor"-Stabilized DNA Nanostructures.

Autor: Cassinelli V; baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany).; Department Chemistry and Biochemistry, Ludwig-Maximilians-Universität (LMU), Butenandtstrasse 5-13, 81377 Munich (Germany)., Oberleitner B; baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany)., Sobotta J; baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany).; Department Applied Chemistry, Technische Hochschule Nürnberg G. S. Ohm, Kesslerplatz 12, 90489 Nürnberg (Germany)., Nickels P; Physics Department and CeNS, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539 Munich (Germany)., Grossi G; Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus (Denmark)., Kempter S; Physics Department and CeNS, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539 Munich (Germany)., Frischmuth T; baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany)., Liedl T; Physics Department and CeNS, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539 Munich (Germany)., Manetto A; baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany). a.manetto@baseclick.eu.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2015 Jun 26; Vol. 54 (27), pp. 7795-8. Date of Electronic Publication: 2015 May 15.
DOI: 10.1002/anie.201500561
Abstrakt: DNA-based self-assembled nanostructures are widely used to position organic and inorganic objects with nanoscale precision. A particular promising application of DNA structures is their usage as programmable carrier systems for targeted drug delivery. To provide DNA-based templates that are robust against degradation at elevated temperatures, low ion concentrations, adverse pH conditions, and DNases, we built 6-helix DNA tile tubes consisting of 24 oligonucleotides carrying alkyne groups on their 3'-ends and azides on their 5'-ends. By a mild click reaction, the two ends of selected oligonucleotides were covalently connected to form rings and interlocked DNA single strands, so-called DNA catenanes. Strikingly, the structures stayed topologically intact in pure water and even after precipitation from EtOH. The structures even withstood a temperature of 95 °C when all of the 24 strands were chemically interlocked.
(© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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