Comprehensive analysis of the venom gland transcriptome of the spider Dolomedes fimbriatus.

Autor:	Kozlov SA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ul. Miklukho-Maklaya, 16/10 , Moscow 117997, Russia., Lazarev VN; Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia ; Moscow Institute of Physics and Technology, Dolgoprudny , Moscow Region 141700, Russia., Kostryukova ES; Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia ; Moscow Institute of Physics and Technology, Dolgoprudny , Moscow Region 141700, Russia., Selezneva OV; Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia., Ospanova EA; Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia., Alexeev DG; Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia ; Moscow Institute of Physics and Technology, Dolgoprudny , Moscow Region 141700, Russia., Govorun VM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ul. Miklukho-Maklaya, 16/10 , Moscow 117997, Russia ; Scientific Research Institute of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russian Federation, 1a, Malaya Pirogovskaya st. , Moscow 119435, Russia ; Moscow Institute of Physics and Technology, Dolgoprudny , Moscow Region 141700, Russia., Grishin EV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ul. Miklukho-Maklaya, 16/10 , Moscow 117997, Russia.
Jazyk:	angličtina
Zdroj:	Scientific data [Sci Data] 2014 Aug 05; Vol. 1, pp. 140023. Date of Electronic Publication: 2014 Aug 05 (Print Publication: 2014).
DOI:	10.1038/sdata.2014.23
Abstrakt:	A comprehensive transcriptome analysis of an expressed sequence tag (EST) database of the spider Dolomedes fimbriatus venom glands using single-residue distribution analysis (SRDA) identified 7,169 unique sequences. Mature chains of 163 different toxin-like polypeptides were predicted on the basis of well-established methodology. The number of protein precursors of these polypeptides was appreciably numerous than the number of mature polypeptides. A total of 451 different polypeptide precursors, translated from 795 unique nucleotide sequences, were deduced. A homology search divided the 163 mature polypeptide sequences into 16 superfamilies and 19 singletons. The number of mature toxins in a superfamily ranged from 2 to 49, whereas the diversity of the original nucleotide sequences was greater (2-261 variants). We observed a predominance of inhibitor cysteine knot toxin-like polypeptides among the cysteine-containing structures in the analyzed transcriptome bank. Uncommon spatial folds were also found.
Databáze:	MEDLINE
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