| Autor: |
Squaiella-Baptistão CC; Laboratório de Imunoquímica, Instituto Butantan, Avenida Vital Brazil 1500, Prédio Novo, 2° Andar, Butantã, 05503-900 São Paulo, SP, Brazil., Teixeira D; Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu 862, Edifício de Ciências Biomédicas, 4° Andar, Vila Clementino, 04023-062 São Paulo, SP, Brazil., Mussalem JS; Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu 862, Edifício de Ciências Biomédicas, 4° Andar, Vila Clementino, 04023-062 São Paulo, SP, Brazil., Ishimura ME; Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu 862, Edifício de Ciências Biomédicas, 4° Andar, Vila Clementino, 04023-062 São Paulo, SP, Brazil., Longo-Maugéri IM; Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu 862, Edifício de Ciências Biomédicas, 4° Andar, Vila Clementino, 04023-062 São Paulo, SP, Brazil. |
| Abstrakt: |
Propionibacterium acnes (P. acnes) is a gram-positive anaerobic bacillus present in normal human skin microbiota, which exerts important immunomodulatory effects, when used as heat- or phenol-killed suspensions. We previously demonstrated that heat-killed P. acnes or its soluble polysaccharide (PS), extracted from the bacterium cell wall, suppressed or potentiated the Th2 response to ovalbumin (OVA) in an immediate hypersensitivity model, depending on the treatment protocol. Herein, we investigated the mechanisms responsible for these effects, using the same model and focusing on the activation status of antigen-presenting cells (APCs). We verified that higher numbers of APCs expressing costimulatory molecules and higher expression levels of these molecules are probably related to potentiation of the Th2 response to OVA induced by P. acnes or PS, while higher expression of toll-like receptors (TLRs) seems to be related to Th2 suppression. In vitro cytokines production in cocultures of dendritic cells and T lymphocytes indicated that P. acnes and PS seem to perform their effects by acting directly on APCs. Our data suggest that P. acnes and PS directly act on APCs, modulating the expression of costimulatory molecules and TLRs, and these differently activated APCs drive distinct T helper patterns to OVA in our model. |
