Improved antibacterial phototoxicity of a neutral porphyrin in natural deep eutectic solvents.

Autor: Wikene KO; Department of Pharmacy, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway. Electronic address: k.o.wikene@farmasi.uio.no., Bruzell E; Nordic Institute of Dental Materials AS, Sognsveien 70A, 0855 Oslo, Norway. Electronic address: ellen.bruzell@niom.no., Tønnesen HH; Department of Pharmacy, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway. Electronic address: h.h.tonnesen@farmasi.uio.no.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2015 Jul; Vol. 148, pp. 188-196. Date of Electronic Publication: 2015 May 02.
DOI: 10.1016/j.jphotobiol.2015.04.022
Abstrakt: Neutral porphyrins for antibacterial photodynamic therapy (aPDT) have received little attention due to their tendency to aggregate in aqueous media and reports of low phototoxic effect. These compounds may be less toxic to cells than positively and negatively charged photosensitisers. The preparation of highly bacterial phototoxic formulations of neutral porphyrins remains an open field of research with great potential if achievable. The purpose of this study was to develop novel hydrophilic formulations of the neutral porphyrin 5,10,15,20-tetrakis(4-hydroxyphenyl)-porphyrin (THPP) by use of natural deep eutectic solvents (NADES) prepared by the solvent evaporation method. Physical and photochemical stability and in vitro photoinactivation of Enterococcus faecalis and Escherichia coli were investigated. Two of the 15 NADES investigated demonstrated superior solubilising properties of THPP. The photostability of THPP was higher in NADES than in methanol. A 100-fold dilution of the preparations with buffer to a final concentration of 0.5-5 nM THPP resulted in complete photoinactivation of E. faecalis and E. coli both in their exponential and stationary phase. THPP demonstrated significantly higher phototoxicity when formulated in NADES than in other aqueous preparations like phosphate buffered saline. NADES as a formulation concept for photosensitisers shows a great potential in aPDT.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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