Functional G894T (rs1799983) polymorphism and intron-4 VNTR variant of nitric oxide synthase (NOS3) gene are susceptibility biomarkers of obesity among Tunisians.

Autor: Nasr HB; Unité de Recherche UR12ESO6, Physiologie de l'Exercice et Physiopathologie: de l'Intégré au Moléculaire «Biologie, Medecine et Santé», Faculté de Medecine Ibn el Jazzar, Sousse, Tunisia., Dimassi S; Unité de Recherche UR12ESO6, Physiologie de l'Exercice et Physiopathologie: de l'Intégré au Moléculaire «Biologie, Medecine et Santé», Faculté de Medecine Ibn el Jazzar, Sousse, Tunisia., M'hadhbi R; Unité de Recherche UR12ESO6, Physiologie de l'Exercice et Physiopathologie: de l'Intégré au Moléculaire «Biologie, Medecine et Santé», Faculté de Medecine Ibn el Jazzar, Sousse, Tunisia., Debbabi H; Unité de Recherche UR12ESO6, Physiologie de l'Exercice et Physiopathologie: de l'Intégré au Moléculaire «Biologie, Medecine et Santé», Faculté de Medecine Ibn el Jazzar, Sousse, Tunisia., Kortas M; Laboratoire d'Hématologie, CHU Farhat Hached, Sousse, Tunisia., Tabka Z; Unité de Recherche UR12ESO6, Physiologie de l'Exercice et Physiopathologie: de l'Intégré au Moléculaire «Biologie, Medecine et Santé», Faculté de Medecine Ibn el Jazzar, Sousse, Tunisia., Chahed K; Unité de Recherche UR12ESO6, Physiologie de l'Exercice et Physiopathologie: de l'Intégré au Moléculaire «Biologie, Medecine et Santé», Faculté de Medecine Ibn el Jazzar, Sousse, Tunisia; Département de Biologie, Faculté des Sciences de Sfax, Tunisia. Electronic address: k.chahed@yahoo.fr.
Jazyk: angličtina
Zdroj: Obesity research & clinical practice [Obes Res Clin Pract] 2016 Jul-Aug; Vol. 10 (4), pp. 465-75. Date of Electronic Publication: 2015 May 05.
DOI: 10.1016/j.orcp.2015.04.008
Abstrakt: Objective: The endothelial nitric oxide synthase (NOS3) has been shown to play a role in the modulation of lipolysis. The goal of this study was to examine the impact of the G894T (rs1799983) and a 27 bp variable number of tandem repeats (VNTR 4a/b) of NOS3 gene on obesity in a sample of the Tunisian population.
Research Methods and Procedures: The study included 211 normal weight subjects and 183 obese patients. NOS3 G894T and 4a/b variants were determined by PCR analysis and examined for association with obesity-related traits. The effect of obesity on forearm skin blood flow (FSBF) response to acetylcholine, an endothelium-dependent vasodilator was determined by laser Doppler iontophoresis.
Results: In case-control studies, both G894T and 4a/b variants were associated with obesity. A significantly increased risk of obesity was found with the NOS3(G894T) TT genotype (OR:2.62, P=0.04). This association remains significant after adjustments for age and gender (OR: 2.93, P=0.03). A higher risk was also observed for carriers of the G894T allele (OR: 1.72, P=0.001). Stratified analysis by gender revealed that obese men (but not women) had significantly higher frequency of TT genotypes compared to controls (9.9% vs. 2.9%, P=0.01). Carriers of the 4b allele presented a significantly higher risk of obesity than non-carriers even after adjustments for age and gender (OR (95%CI): 1.72 (1.16-2.56), P=0.004). Correlations with anthropometric parameters revealed that carriers of TT and bb genotypes had significantly higher body mass index compared to those homozygous for the G and a alleles (P=0.0004).
Conclusion: This study provides the first evidence for the association of G894T and 4a/b variants with body mass index and the risk of obesity in Tunisians. These polymorphisms did not exhibit, however any significant association with both metabolic traits and vascular function.
(Copyright © 2015 Asia Oceania Association for the Study of Obesity. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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