Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure.

Autor:	Webber MA; Antimicrobials Research Group, School of Immunity and Infection and Institute for Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK m.a.webber@bham.ac.uk., Whitehead RN; Antimicrobials Research Group, School of Immunity and Infection and Institute for Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK., Mount M; Antimicrobials Research Group, School of Immunity and Infection and Institute for Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK., Loman NJ; School of Bioscience and Institute for Microbiology & Infection, College of Life and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UK., Pallen MJ; Division of Microbiology and Infection, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK., Piddock LJ; Antimicrobials Research Group, School of Immunity and Infection and Institute for Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK.
Jazyk:	angličtina
Zdroj:	The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2015 Aug; Vol. 70 (8), pp. 2241-8. Date of Electronic Publication: 2015 May 07.
DOI:	10.1093/jac/dkv109
Abstrakt:	Objectives: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance. Methods: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance. Results: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan. Conclusions: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens. (© The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.)
Databáze:	MEDLINE
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