The changes in miR-130b levels in human serum and the correlation with the severity of diabetic nephropathy.

Autor: Lv C; Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China., Zhou YH; Division of Endocrinology, Shenyang No. 8 Hospital, Shenyang, Liaoning, 110024, China., Wu C; Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China., Shao Y; Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China., Lu CL; Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China., Wang QY; Department of Endocrinology, the First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001, China.
Jazyk: angličtina
Zdroj: Diabetes/metabolism research and reviews [Diabetes Metab Res Rev] 2015 Oct; Vol. 31 (7), pp. 717-24. Date of Electronic Publication: 2015 Jun 25.
DOI: 10.1002/dmrr.2659
Abstrakt: Background: Circulating microRNA 130b has been closely associated with multiple diseases in humans such as cancer, obesity and diabetes mellitus. This study evaluates the correlation between serum miR-130b and the severity of diabetic nephropathy evaluated by measurement of albuminuria.
Methods: Three hundred twenty-seven patients with type 2 diabetes mellitus (T2DM) were divided into three groups: normoalbuminuria group [diabetes mellitus, urinary albumin to creatinine ratio (UACR) < 30 mg/g, n = 137], microalbuminuria group (DN1, UACR 30-300 mg/g, n = 122) and macroalbuminuria group (DN2, UACR > 300 mg/g, n = 68). The levels of serum miR-130b were validated by real-time polymerase chain reaction. Serum transforming growth factor β1 (TGF-β1), hypoxia inducible factor 1α (HIF-1α) and fibronectin were determined by enzyme-linked immunosorbent assay.
Results: Compared with control, serum miR-130b levels were significantly decreased in T2DM patients and further decreased in the patients of diabetes mellitus, DN1 and DN2 groups (p < 0.001). Furthermore, age-adjusted and sex-adjusted regression analyses showed that decreased level of serum miR-130b, increased levels of glycated haemoglobin (HbA1c ), homeostatic model assessment of insulin resistance (HOMA-IR), triglyceride (TG), low-density lipoprotein (LDL), serum creatinine, blood urea nitrogen (BUN), TGF-β1, HIF-1α and fibronectin were significantly correlated with UACR (p < 0.05). In addition, serum miR-130b levels were inversely correlated with HbA1c , HOMA-IR, TG, LDL, BUN, TGF-β1, HIF-1α and FN (p < 0.05).
Conclusion: Our findings suggest that serum miR-130b may be a new biomarker for the early diagnosis of DN in T2DM. Circulating miR-130b may possibly be involved in the pathological mechanism of DN, such as lipid metabolic disorders, oxidative stress, extracellular matrix deposition and renal fibrosis.
(Copyright © 2015 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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