| Autor: |
Permyakova NV; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia., Zagorskaya AA; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia., Belavin PA; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia., Uvarova EA; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia., Nosareva OV; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia ; State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk 630559, Russia., Nesterov AE; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia ; State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk 630559, Russia., Novikovskaya AA; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia., Zav'yalov EL; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia., Moshkin MP; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia., Deineko EV; Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia. |
| Abstrakt: |
Tuberculosis remains one of the major infectious diseases, which continues to pose a major global health problem. Transgenic plants may serve as bioreactors to produce heterologous proteins including antibodies, antigens, and hormones. In the present study, a genetic construct has been designed that comprises the Mycobacterium tuberculosis genes cfp10, esat6 and dIFN gene, which encode deltaferon, a recombinant analog of the human γ-interferon designed for expression in plant tissues. This construct was transferred to the carrot (Daucus carota L.) genome by Agrobacterium-mediated transformation. This study demonstrates that the fusion protein CFP10-ESAT6-dIFN is synthesized in the transgenic carrot storage roots. The protein is able to induce both humoral and cell-mediated immune responses in laboratory animals (mice) when administered either orally or by injection. It should be emphasized that M. tuberculosis antigens contained in the fusion protein have no cytotoxic effect on peripheral blood mononuclear cells. |
