| Autor: |
Uzarski JS; 1 Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine , Chicago, Illinois.; 2 Department of Surgery, Northwestern University Feinberg School of Medicine , Chicago, Illinois., Bijonowski BM; 1 Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine , Chicago, Illinois.; 2 Department of Surgery, Northwestern University Feinberg School of Medicine , Chicago, Illinois., Wang B; 1 Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine , Chicago, Illinois.; 2 Department of Surgery, Northwestern University Feinberg School of Medicine , Chicago, Illinois., Ward HH; 3 Department of Internal Medicine, University of New Mexico HSC , Albuquerque, New Mexico., Wandinger-Ness A; 4 Department of Pathology, University of New Mexico HSC , Albuquerque, New Mexico., Miller WM; 5 Department of Chemical and Biological Engineering, Northwestern University , Evanston, Illinois.; 6 Chemistry of Life Processes Institute, Northwestern University , Evanston, Illinois., Wertheim JA; 1 Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine , Chicago, Illinois.; 2 Department of Surgery, Northwestern University Feinberg School of Medicine , Chicago, Illinois.; 6 Chemistry of Life Processes Institute, Northwestern University , Evanston, Illinois.; 7 Department of Surgery, Jesse Brown VA Medical Center , Chicago, Illinois.; 8 Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University , Chicago, Illinois.; 9 Department of Biomedical Engineering, Northwestern University , Evanston, Illinois. |
| Abstrakt: |
Analysis of perfusion-based bioreactors for organ engineering and a detailed evaluation of physical and biochemical parameters that measure dynamic changes within maturing cell-laden scaffolds are critical components of ex vivo tissue development that remain understudied topics in the tissue and organ engineering literature. Intricately designed bioreactors that house developing tissue are critical to properly recapitulate the in vivo environment, deliver nutrients within perfused media, and monitor physiological parameters of tissue development. Herein, we provide an in-depth description and analysis of two dual-purpose perfusion bioreactors that improve upon current bioreactor designs and enable comparative analyses of ex vivo scaffold recellularization strategies and cell growth performance during long-term maintenance culture of engineered kidney or liver tissues. Both bioreactors are effective at maximizing cell seeding of small-animal organ scaffolds and maintaining cell survival in extended culture. We further demonstrate noninvasive monitoring capabilities for tracking dynamic changes within scaffolds as the native cellular component is removed during decellularization and model human cells are introduced into the scaffold during recellularization and proliferate in maintenance culture. We found that hydrodynamic pressure drop (ΔP) across the retained scaffold vasculature is a noninvasive measurement of scaffold integrity. We further show that ΔP, and thus resistance to fluid flow through the scaffold, decreases with cell loss during decellularization and correspondingly increases to near normal values for whole organs following recellularization of the kidney or liver scaffolds. Perfused media may be further sampled in real time to measure soluble biomarkers (e.g., resazurin, albumin, or kidney injury molecule-1) that indicate degree of cellular metabolic activity, synthetic function, or engraftment into the scaffold. Cell growth within bioreactors is validated for primary and immortalized cells, and the design of each bioreactor is scalable to accommodate any three-dimensional scaffold (e.g., synthetic or naturally derived matrix) that contains conduits for nutrient perfusion to deliver media to growing cells and monitor noninvasive parameters during scaffold repopulation, broadening the applicability of these bioreactor systems. |
