A Luciferase Reporter Gene Assay to Measure Ebola Virus Viral Protein 35-Associated Inhibition of Double-Stranded RNA-Stimulated, Retinoic Acid-Inducible Gene 1-Mediated Induction of Interferon β.

Autor:	Cannas V; Department of Life and Environmental Sciences, University of Cagliari, Italy., Daino GL; Department of Life and Environmental Sciences, University of Cagliari, Italy., Corona A; Department of Life and Environmental Sciences, University of Cagliari, Italy., Esposito F; Department of Life and Environmental Sciences, University of Cagliari, Italy., Tramontano E; Department of Life and Environmental Sciences, University of Cagliari, Italy.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2015 Oct 01; Vol. 212 Suppl 2, pp. S277-81. Date of Electronic Publication: 2015 Apr 29.
DOI:	10.1093/infdis/jiv214
Abstrakt:	During Ebola virus (EBOV) infection, the type I interferon α/β (IFN-α/β) innate immune response is suppressed by EBOV viral protein 35 (VP35), a validated drug target. Identification of EBOV VP35 inhibitors requires a cellular system able to assess the VP35-based inhibitory functions of viral double-stranded RNA (dsRNA) IFN-β induction. We established a miniaturized luciferase gene reporter assay in A549 cells that measures IFN-β induction by viral dsRNA and is dose-dependently inhibited by VP35 expression. When compared to influenza A virus NS1 protein, EBOV VP35 showed improved inhibition of viral dsRNA-based IFN-β induction. This assay can be used to screen for EBOV VP35 inhibitors. (© The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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