| Autor: |
Ting SY; 1 Department of Mechanical Engineering, The University of Tokyo , Tokyo, Japan ., Montagne K; 1 Department of Mechanical Engineering, The University of Tokyo , Tokyo, Japan ., Nishimura Y; 2 Sigmax , Tokyo, Japan ., Ushida T; 1 Department of Mechanical Engineering, The University of Tokyo , Tokyo, Japan .; 3 Department of Bioengineering, The University of Tokyo , Tokyo, Japan ., Furukawa KS; 1 Department of Mechanical Engineering, The University of Tokyo , Tokyo, Japan .; 3 Department of Bioengineering, The University of Tokyo , Tokyo, Japan . |
| Jazyk: |
angličtina |
| Zdroj: |
Tissue engineering. Part C, Methods [Tissue Eng Part C Methods] 2015 Oct; Vol. 21 (10), pp. 1005-14. Date of Electronic Publication: 2015 Jun 11. |
| DOI: |
10.1089/ten.TEC.2014.0428 |
| Abstrakt: |
The aim of this study was to evaluate how low-intensity pulsed ultrasound (LIPUS) modulates the effect of transforming growth factor-β3 (TGF-β3) on the differentiation of scaffold-free dedifferentiated bovine articular chondrocyte tissues toward a cartilage-like phenotype. Specifically, the effect of these stimuli on the expression of hypertrophic markers collagen type I, collagen type X, and cartilage-degrading collagenase gene expression for a scaffold-free model was analyzed. A bioreactor that applied LIPUS directly from the transducer through a silicone gel to a six-well plate containing the tissues allowed simple, sterile, and large-scale experiments. Tissues were subjected to LIPUS of 55 mW/cm(2) in a 200 μs burst sine wave of 1 MHz over a 10-day period with or without TGF-β3 (10 ng/mL). Tissues exposed to TGF-β3 had significantly increased glycosaminoglycan and total collagen protein production along with upregulated cartilage-specific gene expression, resulting in tissues with a higher Young's Modulus. However, these tissues had also upregulated gene expression for hypertrophic markers collagen type I, collagen type X, MMP-1, MMP-13, MMP-2, and also an increase in the phosphorylation of p38. The expression of these matrix-degrading enzymes was remediated by hypertrophic development and differentiate dedifferentiated bovine articular chondrocytes towards a chondrogenic lineage allowing it to be a valuable tool in cartilage tissue engineering. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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