G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.

Autor: Macaulay IC; Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK., Haerty W; MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Kumar P; Department of Human Genetics, University of Leuven, Leuven, Belgium., Li YI; MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Hu TX; MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Teng MJ; Cancer Genome Project, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Goolam M; Department of Physiology, Development and Neuroscience, Downing Site, University of Cambridge, Cambridge, UK., Saurat N; Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK., Coupland P; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Shirley LM; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Smith M; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Van der Aa N; Department of Human Genetics, University of Leuven, Leuven, Belgium., Banerjee R; Cytogenetics Core Facility, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Ellis PD; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Quail MA; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Swerdlow HP; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Zernicka-Goetz M; Department of Physiology, Development and Neuroscience, Downing Site, University of Cambridge, Cambridge, UK., Livesey FJ; Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK., Ponting CP; 1] Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK. [2] MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Voet T; 1] Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK. [2] Department of Human Genetics, University of Leuven, Leuven, Belgium.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2015 Jun; Vol. 12 (6), pp. 519-22. Date of Electronic Publication: 2015 Apr 27.
DOI: 10.1038/nmeth.3370
Abstrakt: The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.
Databáze: MEDLINE