Mutagenicity monitoring following battlefield exposures: Molecular analysis of HPRT mutations in Gulf War I veterans exposed to depleted uranium.

Autor: Nicklas JA; Department of Pediatrics, University of Vermont College of Medicine, Burlington, Vermont., Albertini RJ; Department of Pathology, University of Vermont College of Medicine, Burlington, Vermont.; Biostatistics Unit, University of Vermont College of Medicine, Burlington, Vermont., Vacek PM; Center for Clinical and Translational Science - Biomedical Informatics Unit, University of Vermont, Burlington, Vermont., Ardell SK; Division of Newborn Medicine, Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania., Carter EW; Center for Clinical and Translational Science - Biomedical Informatics Unit, University of Vermont, Burlington, Vermont., McDiarmid MA; Occupational Health Program, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland., Engelhardt SM; Department of Veterans Affairs Medical Center, Baltimore, Maryland., Gucer PW; Occupational Health Program, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland., Squibb KS; Occupational Health Program, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland.
Jazyk: angličtina
Zdroj: Environmental and molecular mutagenesis [Environ Mol Mutagen] 2015 Aug; Vol. 56 (7), pp. 594-608. Date of Electronic Publication: 2015 May 12.
DOI: 10.1002/em.21956
Abstrakt: Molecular studies that involved cDNA and genomic DNA sequencing as well as multiplex PCR of the HPRT gene were performed to determine the molecular mutational spectrum for 1,377 HPRT mutant isolates obtained from 61 Veterans of the 1991 Gulf War, most of whom were exposed to depleted uranium (DU). Mutant colonies were isolated from one to four times from each Veteran (in 2003, 2005, 2007, and/or 2009). The relative frequencies of the various types of mutations (point mutations, deletions, insertions, etc.) were compared between high versus low DU exposed groups, (based on their urine U concentration levels), with HPRT mutant frequency (as determined in the companion paper) and with a database of historic controls. The mutational spectrum includes all classes of gene mutations with no significant differences observed in Veterans related to their DU exposures.
(© 2015 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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