Rapid and reliable discrimination between Shigella species and Escherichia coli using MALDI-TOF mass spectrometry.

Autor: Paauw A; Department of CBRN Protection, TNO, PO Box 45, 2280 AA Rijswijk, The Netherlands. Electronic address: armand.paauw@tno.nl., Jonker D; Cluster of Infectious Diseases, Public Health Service Amsterdam, PO Box 2200, 1000 CE Amsterdam, The Netherlands., Roeselers G; Department of CBRN Protection, TNO, PO Box 45, 2280 AA Rijswijk, The Netherlands., Heng JM; Department of CBRN Protection, TNO, PO Box 45, 2280 AA Rijswijk, The Netherlands., Mars-Groenendijk RH; Department of CBRN Protection, TNO, PO Box 45, 2280 AA Rijswijk, The Netherlands., Trip H; Department of CBRN Protection, TNO, PO Box 45, 2280 AA Rijswijk, The Netherlands., Molhoek EM; Department of CBRN Protection, TNO, PO Box 45, 2280 AA Rijswijk, The Netherlands., Jansen HJ; Expert Centre Force Health Protection, Ministry of Defence, PO Box 185, MPC 56A, 3940 AD, Doorn, The Netherlands., van der Plas J; Expert Centre Force Health Protection, Ministry of Defence, PO Box 185, MPC 56A, 3940 AD, Doorn, The Netherlands., de Jong AL; Department of CBRN Protection, TNO, PO Box 45, 2280 AA Rijswijk, The Netherlands., Majchrzykiewicz-Koehorst JA; Department of CBRN Protection, TNO, PO Box 45, 2280 AA Rijswijk, The Netherlands., Speksnijder AG; Cluster of Infectious Diseases, Public Health Service Amsterdam, PO Box 2200, 1000 CE Amsterdam, The Netherlands; Naturalis Biodiversity Centre, Sylviusweg 72, 2333 BE Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: International journal of medical microbiology : IJMM [Int J Med Microbiol] 2015 Jun-Aug; Vol. 305 (4-5), pp. 446-52. Date of Electronic Publication: 2015 Apr 13.
DOI: 10.1016/j.ijmm.2015.04.001
Abstrakt: E. coli-Shigella species are a cryptic group of bacteria in which the Shigella species are distributed within the phylogenetic tree of E. coli. The nomenclature is historically based and the discrimination of these genera developed as a result of the epidemiological need to identify the cause of shigellosis, a severe disease caused by Shigella species. For these reasons, this incorrect classification of shigellae persists to date, and the ability to rapidly characterize E. coli and Shigella species remains highly desirable. Until recently, existing matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) assays used to identify bacteria could not discriminate between E. coli and Shigella species. Here we present a rapid classification method for the E. coli-Shigella phylogroup based on MALDI-TOF MS which is supported by genetic analysis. E. coli and Shigella isolates were collected and genetically characterized by MLVA. A custom reference library for MALDI-TOF MS that represents the genetic diversity of E. coli and Shigella strains was developed. Characterization of E. coli and Shigella species is based on an approach with Biotyper software. Using this reference library it was possible to distinguish between Shigella species and E. coli. Of the 180 isolates tested, 94.4% were correctly classified as E. coli or shigellae. The results of four (2.2%) isolates could not be interpreted and six (3.3%) isolates were classified incorrectly. The custom library extends the existing MALDI-TOF MS method for species determination by enabling rapid and accurate discrimination between Shigella species and E. coli.
(Copyright © 2015 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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