Characterization of Gastric Cardia Tumors: Differences in Helicobacter pylori Strains and Genetic Polymorphisms.

Autor: da Costa DM; Molecular Genetics Laboratory, Department of Pathology and Forensic Medicine, School of Medicine, Federal University of Ceará, Street Coronel Nunes de Melo, 1315 - Rodolfo Teófilo, Fortaleza, Ceará, 60430-270, Brazil, debora_mcosta@hotmail.com., Dos Santos Pereira E, de Lima Silva-Fernandes IJ, Ferreira MV, Rabenhorst SH
Jazyk: angličtina
Zdroj: Digestive diseases and sciences [Dig Dis Sci] 2015 Sep; Vol. 60 (9), pp. 2712-7. Date of Electronic Publication: 2015 Apr 24.
DOI: 10.1007/s10620-015-3666-0
Abstrakt: Background: Gastric cancer results from a multifactorial process and is one of the most common causes of death worldwide. These tumors can arise in the distal stomach (non-cardia) and in the cardia region, presenting different characteristics and frequency of occurrence worldwide.
Aims: To search for differences between tumors of different locations that could explain the presence of cardia tumors, considering Helicobacter pylori strains and genetic polymorphisms.
Materials and Methods: DNA was extracted from gastric adenocarcinoma tissue of 127 patients. Helicobacter pylori genes were detected by PCR, and polymorphisms by PCR-RFLP.
Results: Most of the tumors were located in non-cardia. The genotype 28152GA of XRCC1 showed an increase in risk of cardia tumors. In analysis performed considering gender, women carrying TNF-308GA genotype showed a decreased risk of non-cardia tumors, while in men the decreased risk of non-cardia tumors was associated with TNF-308GG genotype. Genotypes combinations showed that the SNPs RAD51 135G>C, XRCC3 18067C>T, and XRCC1 28152G>A had some combinations more frequent in cardia tumors, with an increased risk. Patients infected by cagE-positive strains presented a positive correlation with non-cardia tumors.
Conclusion: The results showed some susceptibility differences between tumors of different locations. There was an increased risk relationship between three repair enzyme SNPs and cardia tumors, and the G allele of the cytokine gene TNF negatively influenced the development of non-cardia tumors. Helicobacter pylori strains seemed to be different in the cardia region, where they were less virulent than those located in the distal region of the stomach.
Databáze: MEDLINE
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