Genome and proteome analysis of Pseudomonas chloritidismutans AW-1 T that grows on n-decane with chlorate or oxygen as electron acceptor.

Autor: Mehboob F; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands., Oosterkamp MJ; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands., Koehorst JJ; Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands., Farrakh S; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands., Veuskens T; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands., Plugge CM; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands., Boeren S; Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, Wageningen, 6703 HA, The Netherlands., de Vos WM; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands., Schraa G; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands., Stams AJ; Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands.; Centre of Biological Engineering, University of Minho, Braga, 4710-057, Portugal., Schaap PJ; Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, Wageningen, 6703 HB, The Netherlands. Peter.Schaap@wur.nl.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2016 Oct; Vol. 18 (10), pp. 3247-3257. Date of Electronic Publication: 2015 Jun 05.
DOI: 10.1111/1462-2920.12880
Abstrakt: Growth of Pseudomonas chloritidismutans AW-1 T on C7 to C12 n-alkanes with oxygen or chlorate as electron acceptor was studied by genome and proteome analysis. Whole genome shotgun sequencing resulted in a 5 Mbp assembled sequence with a G + C content of 62.5%. The automatic annotation identified 4767 protein-encoding genes and a putative function could be assigned to almost 80% of the predicted proteins. The distinct phylogenetic position of P. chloritidismutans AW-1 T within the Pseudomonas stutzeri cluster became clear by comparison of average nucleotide identity values of sequenced genomes. Analysis of the proteome of P. chloritidismutans AW-1 T showed the versatility of this bacterium to adapt to aerobic and anaerobic growth conditions with acetate or n-decane as substrates. All enzymes involved in the alkane oxidation pathway were identified. An alkane monooxygenase was detected in n-decane-grown cells, but not in acetate-grown cells. The enzyme was found when grown in the presence of oxygen or chlorate, indicating that under both conditions an oxygenase-mediated pathway is employed for alkane degradation. Proteomic and biochemical data also showed that both chlorate reductase and chlorite dismutase are constitutively present, but most abundant under chlorate-reducing conditions.
(© 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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